Journal: Frontiers in Immunology

Article Title: Unveiling B7/CD28 family proteins in hepatocellular carcinoma: insights into communication and prognostic significance

doi: 10.3389/fimmu.2025.1583597

Figure Lengend Snippet: The expression pattern of B7H3 in tumor tissues. (A) The representative IHC images of Isotype control antibody and B7H3 antibody; (B) The representative IHC images of diffuse HCC and well-circumscribed HCC; (C) Representative multi-immunofluorescence images of AFP (green) and B7H3 (red). The nucleus is labeled with DAPI (blue). (D) Proportions of B7H3 on tumor cells (TCs), in stroma and in both. (E) The representative IHC images of B7H3 from serial sections. (F) OS and PFS for HCC patients based on the expression of B7H3 on tumor tissue. (G) OS and PFS for HCC patients based on the expression of B7H3 in the stroma and on tumor cells.

Article Snippet: In the end, the specimens were prepared by applying ProLongTM Diamond Antifade mountant with DAPI (G1236, Servicebio, Wuhan, China).

Techniques: Expressing, Control, Immunofluorescence, Labeling

Journal: The EMBO Journal

Article Title: Functional rescue of a disease-linked ERAD pathway mutation via alternative splicing

doi: 10.1038/s44318-026-00757-5

Figure Lengend Snippet: ( A ) The survival rate of WT, HET and KI pups at postnatal day 0 (p0). Observed vs. Expected * P = 0.013. ( B ) Body weight at p0. n = 7–9 mice/group. WT vs. KI, P = 0.94. ( C ) The photo of pups at postnatal 4 h (upper) and >12 h (lower). Scale bars: 1 cm. ( D ) Tissue morphology of SEL1L C141Y KI pups at P0, with tissue weight quantification shown below. n = 7–9 mice/group. Scale bars: 1 cm. WT vs. KI, Liver P = 0.45, Brain P = 0.65, Thymus P = 0.14, Spleen P = 0.16. ( E ) Morphology of postprandial stomach in the P0 WT and KI pup, with quantification shown on the right. n = 7–9 mice/group. Scale bars : 1 cm. WT vs. KI, **** P = 0.000013. ( F ) The survival curve of WT, HET and KI pups after birth. n = 17–41 mice/group. WT vs. HET, P = 0.86; WT vs. KI, **** P < 0.0001. ( G ) Body weight growth of male and female WT, HET, KI’ mice for postnatal week 1 to week 10. n = 3–20 mice/group. ( H ) Quantification of B cells as a percentage of CD45⁺ peripheral blood mononuclear cells (PBMCs) at p0 by flow cytometry in Fig. . n = 5–10 mice/group. WT vs. KI, *** P = 0.0002; WT vs. KI’, P = 0.63; KI vs. KI’, ** P = 0.0091. ( I , J ) Hematoxylin and eosin (H&E) staining and Immunofluorescence of CD19 + B cells in p0 mouse spleens from SEL1L C141Y KI pups at p0, with quantification shown in ( J ). Red, CD19; Blue, DAPI. n = 3 mice/group. Scale bars: 50 μm for zoom-out photo, 20 μm for zoom-in photo and immunofluorescence figure. *** P = 0.0026. Data are represented as means ± SEM. n.s. not significant. * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001, by Chi-square test for ( A ); two-tailed t test for ( B , D , E , J ); Mantel–Cox test for ( F ); two-way ANOVA followed by Tukey’s multiple-comparisons test for ( G ); one-way ANOVA followed by Tukey’s post hoc test for ( H ). .

Article Snippet: ProLongTM Diamond Antifade Mountant with DAPI , Fisher Scientific , P36971.

Techniques: Flow Cytometry, Staining, Immunofluorescence, Two Tailed Test

Journal: The EMBO Journal

Article Title: Functional rescue of a disease-linked ERAD pathway mutation via alternative splicing

doi: 10.1038/s44318-026-00757-5

Figure Lengend Snippet: ( A – C ) Western blot analysis of SEL1L protein expression ( A ), HRD1 and ERAD substrate IRE1α ( B ) in mouse brains or cortex at indicated ages, with quantification in ( C ). n = 3–4 mice/group. SEL1L: WT vs. HET, P = 0.057; WT vs. KI, *** P = 0.0006; HRD1: WT vs. HET, P = 0.52; WT vs. KI, ** P = 0.0017; IRE1α: WT vs. HET, P = 0.99; WT vs. KI, **** P < 0.0001. ( D , E ) Western blot analysis ( D ) of ERAD protein and ERAD substrates from WT and KI’ mice brain with quantification shown in ( E ). n = 3–4 mice/group. SEL1L: WT vs. KI’, *** P = 0.0009; HRD1: WT vs. KI’, P = 0.34; IRE1α: WT vs. KI’, P = 0.28. ( F ) Phos-tag gel analysis of IRE1α phosphorylation in p0 WT, HET and KI brains. n = 3–4 mice/group. ( G ) RT-PCR analysis of Xbp1 mRNA splicing of p0 WT, KI, and KI’ brains with quantification in Fig. . n = 3–6 mice/group, 3–4 for positive controls. u, unspliced. s, spliced. ( H , I ) Immunofluorescence ( H ) of SATB2+ Layer II/III cells and the CTIP2+ Layer V in the cortex of p0 WT, KI and KI’ mice, with zoom-in region showing SATB2+ cells below Layer V and with quantification shown in ( I ). n = 3–6 mice/group. Green, CTIP2; Red, SATB2; Blue, DAPI. WT vs. KI, * P = 0.046; WT vs. KI’, * P = 0.032; KI vs. KI’, *** P = 0.0007. Data are represented as means ± SEM. n.s. not significant. * P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001, by one-way ANOVA followed by Tukey’s post hoc test for ( C , I ); two-tailed t test for ( E ). .

Article Snippet: ProLongTM Diamond Antifade Mountant with DAPI , Fisher Scientific , P36971.

Techniques: Western Blot, Expressing, Phospho-proteomics, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Two Tailed Test

Journal: Toxics

Article Title: Present in the Aquatic Environment, Unclear Evidence in Top Predators—The Unknown Effects of Anti-Seizure Medication on Eurasian Otters ( Lutra lutra ) from Northern Germany

doi: 10.3390/toxics11040338

Figure Lengend Snippet: Ionized calcium-binding adapter molecule 1 (IBA1) staining of the hippocampal formation of an otter, coronal section, showing the dentate gyrus (dg) ( A ). White rectangle in ( A ) indicates the area of close-up ( B ) showing granular cells (gc). DAPI, 4′, 6-diamidino-2-phenylindole nuclear staining. Scale bar equals 500 µm in ( A ), 100 µm in ( B ).

Article Snippet: After covering with ProLongTM Gold Antifade Mountant with DAPI (Cell Signaling Tech.

Techniques: Binding Assay, Staining